Journal: Turkish Journal of Obstetrics and Gynecology

Article Title: Evaluating midpregnancy Gas6 levels as predictive value for gestational diabetes and birth outcomes

doi: 10.4274/tjod.galenos.2025.95515

Figure Lengend Snippet: ROC curve for the diagnosis of gestational diabetes mellitus based on serum Gas6 levels GAS6: Growth arrest–specific 6 protein, ROC: Receiver operating characteristic

Article Snippet: Gas6 levels were measured using a double antibody sandwich method with a human Gas6 ELISA kit, branded BT Lab Bioassay Technology Laboratory (Cat No. E3257Hu, Shanghai, China).

Techniques: Biomarker Discovery

Journal: Turkish Journal of Obstetrics and Gynecology

Article Title: Evaluating midpregnancy Gas6 levels as predictive value for gestational diabetes and birth outcomes

doi: 10.4274/tjod.galenos.2025.95515

Figure Lengend Snippet:

Article Snippet: Gas6 levels were measured using a double antibody sandwich method with a human Gas6 ELISA kit, branded BT Lab Bioassay Technology Laboratory (Cat No. E3257Hu, Shanghai, China).

Techniques: Biomarker Discovery

Journal: Journal of Nanobiotechnology

Article Title: Microglia-specific interleukin-4 delivery by engineered extracellular vesicles restores inner blood-retinal barrier in diabetic retinopathy via GAS6-MERTK pathway

doi: 10.1186/s12951-025-03976-w

Figure Lengend Snippet: IL4@CHHSSSARC-EV promoted microglial phagocytosis via GAS6-MERTK pathway. A Representative immunofluorescence staining images of IBA1 and C-C3 on retinal flat mounts from OIR mice treated with PBS or IL4@CHHSSSARC-EV. Scale bar: 100 μm. Enlarged: 50 μm. B qPCR analysis of GAS6 and TAM systems (Axl, MERTK, Tyro3) mRNA levels in M1 microglia exposed to PBS or IL4@CHHSSSARC-EV ( n = 3). C Concentrations of GAS6 in the supernatant of M1 microglia after exposed to PBS or IL4@CHHSSSARC-EV by ELISA ( n = 3). D Representative western blot images and quantitative analysis of protein expression of p-MERTK and MERTK after M1 microglia were exposed to PBS or IL4@CHHSSSARC-EV ( n = 3). E Representative immunofluorescence staining images of IBA1 and C-C3 on retinal flat mounts from OIR mice treated with IL4@CHHSSSARC-EV or IL4@CHHSSSARC-EV plus UNC2025. Scale bar: 100 μm. Enlarged: 50 μm. F Representative western blot images and quantitative analysis of protein expression of p-MERTK and MERTK after M1 microglia were exposed to IL4@CHHSSSARC-EV or IL4@CHHSSSARC-EV plus UNC2025 ( n = 3). G Representative immunofluorescence staining images of IB4 in retinas from OIR mice treated with IL4@CHHSSSARC-EV or IL4@CHHSSSARC-EV plus UNC2025 ( n = 5). Scale bar: 500 μm. White region indicated neovascular area and yellow-circulated region marked avascular area. H Quantitative analysis of neovascular area and avascular area of G . * P < 0.1, ** P < 0.01, *** P < 0.001

Article Snippet: The concentration of GAS6 was determined by the mouse GAS6 ELISA kit (EK2384, Multi Sciences).

Techniques: Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Microglia-specific interleukin-4 delivery by engineered extracellular vesicles restores inner blood-retinal barrier in diabetic retinopathy via GAS6-MERTK pathway

doi: 10.1186/s12951-025-03976-w

Figure Lengend Snippet: IL4@CHHSSSARC-EV promoted microglial phagocytosis via GAS6-MERTK pathway. A Representative immunofluorescence staining images of IBA1 and C-C3 on retinal flat mounts from OIR mice treated with PBS or IL4@CHHSSSARC-EV. Scale bar: 100 μm. Enlarged: 50 μm. B qPCR analysis of GAS6 and TAM systems (Axl, MERTK, Tyro3) mRNA levels in M1 microglia exposed to PBS or IL4@CHHSSSARC-EV ( n = 3). C Concentrations of GAS6 in the supernatant of M1 microglia after exposed to PBS or IL4@CHHSSSARC-EV by ELISA ( n = 3). D Representative western blot images and quantitative analysis of protein expression of p-MERTK and MERTK after M1 microglia were exposed to PBS or IL4@CHHSSSARC-EV ( n = 3). E Representative immunofluorescence staining images of IBA1 and C-C3 on retinal flat mounts from OIR mice treated with IL4@CHHSSSARC-EV or IL4@CHHSSSARC-EV plus UNC2025. Scale bar: 100 μm. Enlarged: 50 μm. F Representative western blot images and quantitative analysis of protein expression of p-MERTK and MERTK after M1 microglia were exposed to IL4@CHHSSSARC-EV or IL4@CHHSSSARC-EV plus UNC2025 ( n = 3). G Representative immunofluorescence staining images of IB4 in retinas from OIR mice treated with IL4@CHHSSSARC-EV or IL4@CHHSSSARC-EV plus UNC2025 ( n = 5). Scale bar: 500 μm. White region indicated neovascular area and yellow-circulated region marked avascular area. H Quantitative analysis of neovascular area and avascular area of G . * P < 0.1, ** P < 0.01, *** P < 0.001

Article Snippet: The concentration of GAS6 was determined by the mouse GAS6 ELISA kit (EK2384, Multi Sciences).

Techniques: Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: Endocrinology

Article Title: Polychlorinated Biphenyls Alter Estrogen Receptor β-mediated Epigenetic Regulation, Promoting Endometriosis

doi: 10.1210/endocr/bqaf146

Figure Lengend Snippet: PCB126 elevated levels of GAS6/ESR2 axis to enhance ESR2 activity. (A, B) GAS6 mRNA levels in IHEECs (A) and IHESCs (B) following treatment with various doses of PCB126 or vehicle. (C, D) ESR2 mRNA levels in IHEECs (C) and IHESCs (D) after treatment with increasing doses of PCB126 or vehicle. (E) Assessment of the intrinsic transcriptional activity of ESR2 induced by GAS6. HeLa cells were transiently transfected with an ESR2 expression vector and an ERE-luciferase reporter, followed by treatment with varying concentrations of GAS6 or vehicle for 48 hours. Relative luciferase activity was calculated as the fold change in GAS6-treated vs vehicle-treated cells. * P < .05; ** P < .01; *** P < .001. Abbreviations: ERE, estrogen response element; ESR2, estrogen receptor β; GAS6, growth arrest–specific 6; IHEEC, immortalized human endometrial epithelial cell; IHESC, immortalized human endometrial stromal cell; NS, nonspecific.

Article Snippet: Gene expression levels of GAS6 and ESR2 were quantified using TaqMan probes for GAS6 (Invitrogen, catalog number: Hs01090305_m1) and ESR2 (Invitrogen, catalog number: Hs00230957_m1).

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Luciferase

Journal: Endocrinology

Article Title: Polychlorinated Biphenyls Alter Estrogen Receptor β-mediated Epigenetic Regulation, Promoting Endometriosis

doi: 10.1210/endocr/bqaf146

Figure Lengend Snippet: PCB126 indirectly elevated DNMT3A through the AXL/GAS6/ESR2 axis, enhancing endometriosis progression by altering the immune response. PCB126 exposure elevated GAS6 levels in endometriotic lesions, leading to the activation of AXL signaling. Activated AXL enhanced ESR2 activity, which in turn upregulated DNMT3A expression. Elevated DNMT3A contributed to developing endometriosis-associated inflammatory and immune microenvironment, promoting disease progression. Abbreviations: DNMT3A, DNA methyltransferase 3A; ESR2, estrogen receptor β; GAS6, growth arrest–specific 6.

Article Snippet: Gene expression levels of GAS6 and ESR2 were quantified using TaqMan probes for GAS6 (Invitrogen, catalog number: Hs01090305_m1) and ESR2 (Invitrogen, catalog number: Hs00230957_m1).

Techniques: Activation Assay, Activity Assay, Expressing, Biomarker Discovery